Performance evaluation the new automated Atellica Hema 580 hematology analyzer.

INTRODUCTION: The Atellica Hema (Siemens Healthineers, Tarrytown, NY, USA) is a new generation multi-parameter analyzer for full blood count, 6-part differential and reticulocyte testing by impedance variation and fluorescence flow cytometry. In this study, we verified the whole blood and limited body fluid modes of the Atellica Hema 580. METHODS: We evaluated precision, linearity, carry-over, throughput and performed a method comparison to assess the performance of the Atellica Hema 580. For comparison of the Atellica Hema 580 with the Sysmex XN-1000 (Sysmex, Kobe, Japan), 140 samples from adult and pediatric patients including both normal and abnormal hematology profiles were analyzed in parallel. RESULTS: The Atellica Hema 580 demonstrated acceptable imprecision within the manufacturer's specifications for whole blood and body fluid modes, good linearity for high and low ranges and no significant carryover. The full blood count, differential and reticulocyte correlated well with the Sysmex XN-1000, except for mean cell hemoglobin concentration, basophil and large immature cells. The optical platelet count, reflexed in 34 samples with a platelet count <150 × 109 /l, showed a strong correlation with the fluorescent platelet count on the Sysmex XN-1000. The morphology flagging efficiency was 92% for white blood cells, 95% for red blood cells and 87% for platelets. CONCLUSION: The Atellica Hema 580 showed good analytical performance and workflow efficiency for a wide range of patient samples.

Performance evaluation of the new measurement channels on the automated Sysmex XN-9000 hematology analyzer.

BACKGROUND: The automated Sysmex XN-9000 hematology system has been designed to meet the throughput and efficiency requirements of high volume laboratories with predominantly abnormal samples. New measurement channels have been introduced namely the white cell nucleated (WNR), white cell differential (WDF), white cell precursor (WPC) and fluorescent platelet (PLT-F) channels. METHODS: The performance of the new measurement channels was evaluated with regards to precision, accuracy, linearity, carryover, throughput and stability. 275 slides were assessed for morphology flagging. Adult and pediatric samples with normal and abnormal hematology profiles were included. RESULTS: The XN-9000 demonstrated acceptable imprecision, good linearity for high and low ranges and no carryover. The full blood count and reticulocyte on the XN-9000 correlated well with the reference ADVIA(2)120. The PLT-F (127±84×109/l) compared with the optical platelet count (131±76×109/l) (r=0.97) and the imprecision was <4% on thrombocytopenic samples. The low white blood cell (WBC) mode reported accurate differentials for samples with a WBC count<0.5×109/l (r=0.93). The nucleated red blood cell count from the WNR (1.22±3.96%) showed an excellent correlation with the manual method (1.12±4.79%) (r=0.99). The WPC channel showed 100% sensitivity for the detection of blasts and abnormal lymphocytes. Further, the WPC correctly suppressed the initial blast/abnormal lymphocyte flag in 34% of the reflexed samples. CONCLUSION: The XN-9000 showed enhanced analytical performance and workflow efficiency for a wide range of patient samples which can be attributed to the incorporation of new measurement channels.